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After correction for multiple testing, coumarin remained the most robustly altered metabolite, indicating a modest but statistically supported response to dietary intervention

For the luciferase assays, 5 L of the supernatant was diluted with 95 L PBS (pH 7.4) in white flat-opaque-bottomed 96-well plates (Thermo Fisher Scientific)

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Filtration removes some contaminants but doesn't sterilize

For subcutaneous injections, a standard insulin syringe is ideal